细胞骨架对凝血酶诱导人血小板分泌反应的调节(英文)

本文利用血小板表面外露的GMP-140为血小板分泌反应的特异性标志,通过放射免疫分析法定量测定血小板表面GMP-140分子数,研究了细胞骨架抑制剂对凝血酶诱导血小板分泌反应的影响。结果表明,凝血酶激活使血小板表面GMP-140的外露明显增加,反应迅速,并在一定范围内呈剂量和时间依赖性;而ADP刺激则几乎不引起GMP-140外露的增加。凝血酶激活前加入不同的细胞骨架抑制剂处理可产生不同的效应:细胞松驰素B(肌动蛋白微丝抑制剂)可明显上调凝血酶诱导的GMP-140外露;而秋水仙素(微管抑制剂)则下调GMP-140的外露;两者同时处理仍呈现明显的上调作用。提示凝血酶作为一种强激活剂,不仅可通过受体-G蛋白-第二信使的途径启动血小板分泌反应,而且可能经诱导肌动蛋白微丝的形成对分泌反应起反馈性负调节作用。微管的存在则可能对凝血酶诱导的分泌反应起促进作用。虽然两种细胞骨架的作用相反,但以微丝的作用为主,两者间无相互拮抗现象。     

豌豆外源凝集素基因的克隆及序列分析

从豌豆幼叶分离基因组ＤＮＡ,设计特异引物,用聚合酶链式反应方法扩增出豌豆外源凝集素基因并克隆到Ｅ．ｃｏｌｉ质粒ｐＢｌｕｅｓｃｒｉｐｔＳＫ（＋）的ＥｃｏＲＶ位点。进一步亚克隆至ｐＵＣ１９。序列分析表明,克隆到的片段大小为８３２ｂｐ,包含了豌豆外源凝集素基因完整的编码序列。该基因无内含子,同报道的已知序列相比,其核苷酸序列及推测的氨基酸序列的同源率分别为９９．６％和９８．９％。     

华南胡椒化学成分的研究

从胡椒科植物华南胡椒（Ｐｉｐｅｒ ａｕｓｔｒｏｓｉｎｅｎｓｅ Ｃ．ＤＣ．）中分离出七个成分,经光谱分析和理化常数测定,分别鉴定为Ｎ－ｉｓｏｂｕｔｙｌ－３（３＇,４＇－ｍｅｔｈｙｌｅｎｅｄｉｏｘｙ－５＇－ｍｅｔｈｏｘｙｐｈｅｎｙｌ）－２Ｅ－ｔｒｉｍｏｎｏｉｅｎａｍｉｄｅ（Ｉ）、Ｎ－ｉｓｏｂｕｔｙｌ－７（３＇,４＇－ｍｅｔｙｌｅｎｅｄｉｏｘｙｐｈｅｎｙｌ）－２Ｅ,４Ｅ－ｈｅｐｔａｄｉｅｎａｍｉｄｅ（Ⅱ     

辽宁三角洲区域生态经济分区及其功能分析

应用主成分分析和系统聚类分析方法,将辽河三角洲划分为３个生态经济区和８个生态经济小区．通过对反映系统功能的３个表现属性的分析,评价了辽河三角洲农业生态经济系统的功能状况．     

新的生物杀虫剂螨虫素对棉花、蔬菜害虫的毒力评价及应用技术研究

本文通过生物杀虫剂螨虫素对棉花、蔬菜害虫的毒力评价及田间应用研究表明,它对棉朱砂叶螨和菜青虫两种害虫杀伤力最强,其中LC90分别为0．078ppm和0．013ppm。兼有胃毒、触杀作用,残效期较长,分别达13天和9天。田间小区试验认为防治菜青虫和茄朱砂叶螨以2ppm浓度为宜。防治适期为卵孵化初期或低龄幼(若)虫（螨)期,其防效能达90%以上。该药特点用药量低、效果好、无公害、值得推广。     

The effect of ethanol on lateral and rotational mobility of plasma membrane vesicles isolated from cultured mar 18.5 hybridoma cells

Intramolecular excimer formation of 1,3-di(1-pyrenyl)propane (Py-3-Py) and fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) were used to evaluate the effect of ethanol on the rate and range of the lateral mobility and the range of the rotational mobility of bulk bilayer structures of the plasma membrane vesicles (ATCC-PMV) isolated from cultured hybridoma cells (ATCC TIB 216). In a concentration-dependent manner, ethanol increased the excimer to monomer fluorescence intensity ratio (I/I) of Py-3-Py in the ATCC-PMV and decreased the anisotropy (r), limiting anisotropy (r) and order parameter (S) of DPH in the ATCC-PMV. This indicates that ethanol increased both the lateral and rotational mobility of the probes in the ATCC-PMV. Selective quenching of DPH by trinitrophenyl groups was utilized to examine the range of transbilayer asymmetric rotational diffusion of the ATCC-PMV. The anisotropy (r), limiting anisotropy (r ) and order parameter (S) of DPH in the inner monolayer were 0.024, 0.032, and 0.069, respectively, greater than calculated for the outer monolayer of the ATCC-PMV. Selective quenching of DPH by trinitrophenyl groups was also used to examine the transbilayer asymmetric effects of ethanol on the range of the rotational mobility of the ATCC-PMV. Ethanol had a greater increasing effect on the range of the rotational mobility of the outer monolayer as compared to the inner monolayer of the ATCC-PMV. It has been proven that ethanol exhibits a selective rather than nonselective fluidizing effect within the transbilayer domains of the ATCC-PMV.This paper was supported in part by a research grant from the Korea Science and Engineering Foundation (KOSEF 88-1013-01) and from the Korea Research Foundation (1991–1993).     

Saturated Molecular Map of the Rice Genome Based on an Interspecific Backcross Population

A molecular map has been constructed for the rice genome comprised of 726 markers (mainly restriction fragment length polymorphisms; RFLPs). The mapping population was derived from a backcross between cultivated rice, Oryza sativa, and its wild African relative, Oryza longistaminata. The very high level of polymorphism between these species, combined with the use of polymerase chain reaction-amplified cDNA libraries, contributed to mapping efficiency. A subset of the probes used in this study was previously used to construct an RFLP map derived from an inter subspecific cross, providing a basis for comparison of the two maps and of the relative mapping efficiencies in the two crosses. In addition to the previously described PstI genomic rice library, three cDNA libraries from rice (Oryza), oat (Avena) and barley (Hordeum) were used in this mapping project. Levels of polymorphism detected by each and the frequency of identifying heterologous sequences for use in rice mapping are discussed. Though strong reproductive barriers isolate O. sativa from O. longistaminata, the percentage of markers showing distorted segregation in this backcross population was not significantly different than that observed in an intraspecific F(2) population previously used for mapping. The map contains 1491 cM with an average interval size of 4.0 cM on the framework map, and 2.0 cM overall. A total of 238 markers from the previously described PstI genomic rice library, 250 markers from a cDNA library of rice (Oryza), 112 cDNA markers from oat (Avena), and 20 cDNA markers from a barley (Hordeum) library, two genomic clones from maize (Zea), 11 microsatellite markers, three telomere markers, eleven isozymes, 26 cloned genes, six RAPD, and 47 mutant phenotypes were used in this mapping project. Applications of a molecular map for plant improvement are discussed.     

Production of panose from maltose by intact cells of Aureobasidium pullulans

Panose, a major component of isomalto-oligosaccharides, was selectively produced from maltose using transglucosylation reaction catalyzed by intact cells of Aureobasidium pullulans. When 50 %(w/v) maltose was used as a substrate, the maximum concentration of panose accumulated in the final reaction mixture was about 50 %(w/w) after 120 hr reaction at 55 °C.     

云南西双版纳桑寄生植物传播与鸟的关系研究

在西双版纳地区桑寄生植物的种子是鸟类传播。传播的鸟类主要是啄花鸟科的纯色啄花鸟,红胸啄花鸟,朱背啄花鸟和黄肛啄花鸟等。桑寄生植物种子的传播方式,一是鸟类蚕食除去外果皮的种子或外果皮果肉相互连着的种子,经消化道消化吸收大部分果肉或外果皮后,将种子排出体外,其次是鸟在觅食中遗漏种子进行传播。     

云南阿昌族的红细胞血型分布

调查了１０２名云南阿昌族的ＡＢＯ,ＮＮＳｓ,Ｒｈ和Ｐ系统的红细胞血型,结果表明,阿昌族的基因频率ｐ（０．３８７４）是迄今国内调查过人群中的最高值,Ｅ（０．２４５９）和ＣＤｅ（０．６９３６）基因或染色体频率较高,而Ｓ（０．０６８６）和Ｐ１（０．１０８９）频率较低;Ｍｓ（０．５９５０）连锁率高于Ｎｓ（０．３３５３）;未发现ＳＳ和Ｒｈ（－Ｄ）阴性表现型。